PARP1, APE1, XRCC1, Pol β, FEN1, LIG1/LIG3, EXO1, DNA2, MRE11–RAD50–NBS1, RPA, RAD51, RAD52, BRCA2, RAD54, ATR–ATRIP, 9-1-1
RFC, PCNA, Pol δ, Pol ε, CMG complex, BLM, WRN, UvrD, PIF1
RNA polymerase tracking, transcription factors, nucleosome positioning, chromatin remodelers
DNA/RNA hairpins, G-quadruplexes, FRET, protein folding, multi-fragment binding-affinity comparison
17.9 kb DNA with two nicks 5 kb apart. Stretch it to open a 5 kb ssDNA gap between two dsDNA arms.
One Atto dye marks the gap junction: L = 3' end or R = 5' end.
Fluorophore: L – Atto647N · R – Atto565, both in stock
Other dye? Ask for your fluorophore of choice →
Per vial: 20 C-Trap runs
$450
Two biotinylated handles. Ligate any dsDNA insert between them (Golden Gate compatible).
An optional Atto dye on Handle L or R (7 bp from the overhang) marks the tether direction.
Fluorophore: no dye · L – Atto647N · R – Atto565, all in stock
Other dye? Ask for your fluorophore of choice →
Per vial: 8 reactions (up to 40 C-Trap runs)
$350 no dye · $390 with dye
Handles with long overhangs. Anneal and ligate your ssDNA or RNA oligo.
Fluorophore: no dye, in stock
Need a dye? Ask for your fluorophore of choice →
Per vial: 8 reactions (up to 40 C-Trap runs)
$390
These constructs are the start of a growing catalogue, and we are building it with you.
Don't see your experiment?
Protein folding, DNA curtains, multi-fragment tethers, other lengths or labels: we design the construct with you.
| ssDNA Gap | 6.3 kb Handles | 5 kb Handles | |
|---|---|---|---|
| Length | 17,853 bp | 6,286 bp each | 5,043 bp and 5,039 bp |
| Structure | 5,005 nt gap (bp 6,453–11,457) between 6,452 bp and 6,396 bp dsDNA arms | 2 handles, your dsDNA insert ligated between | 2 handles, your ssDNA/RNA oligo annealed and ligated between |
| Overhangs | — | GTTG (L) and TGGT (R); your insert needs CAAC and ACCA overhangs | 16 nt (L) and 15 nt (R), single-stranded; handles supplied 5′-phosphorylated, no kinase step needed |
| End labels | 3× biotin at each end | 3× biotin, outer end of each handle | 3× biotin, outer end of each handle |
| Dye position | L: 177 bp before the gap (bottom strand). R: 121 bp past the gap (top strand) | Handle L or R, 7 bp from the overhang | — |
| Per vial | 20 C-Trap runs | 8 reactions (up to 40 C-Trap runs) | 8 reactions (up to 40 C-Trap runs) |
| Storage | +4 °C, protect from light | -20 °C, protect from light (dye versions) | -20 °C |
| Price | $450 | $350 no dye · $390 with dye | $390 |
Fluorophores: Atto647N Ex/Em 646/664 nm · Atto565 Ex/Em 563/592 nm
1 C-Trap run = one 500 µl dilution in channel 2, enough to run one experiment on the C-Trap.
Single-molecule biophysics is moving from simple model systems to complex, multi-protein mechanisms. We handle the DNA engineering, so your lab skips weeks of construct troubleshooting and goes straight to the experiment: DNA repair, replication, chromatin and transcription.
Different fluorophore, length, label or geometry: send us the experiment you want to run and we will scope the construct with you.
Order at smeasybio.com or request a quote from msimonetta@smeasybio.com
Shipping: $25 USA · $40 Europe. Prices in USD.
Downloads: Product flyer (PDF)