Home / Optical tweezers / 5 kb Handles for ssDNA/ssRNA
Handles with long overhangs. Anneal and ligate your ssDNA or RNA oligo.
In stock
Per kit: 5 reactions (25 C-Trap runs)
$350
| Kit contents | Handle L-16 nt and Handle R-15 nt: 30 ng/µl, 30 µl each Control hairpin (2 reactions): 12 nM, 10 µl STOP solution 5×: 400 µl |
|---|---|
| Length | Handle L 5,039 bp · Handle R 5,043 bp |
| Structure | 2 handles, your ssDNA or RNA annealed and ligated between |
| Overhangs | Handle L: 16 nt 5′ overhang Handle R: 15 nt 3′ overhang, recessed strand 5′-phosphorylated |
| Your oligo needs | a 5′ phosphate and both flanking sequences (below) |
| End labels | 3× biotin, outer end of each handle |
| Per kit | 5 ligation reactions (25 C-Trap runs) |
| Supplied by the user | 5′-phosphorylated ssDNA or RNA, 10× T4 DNA ligase buffer, T4 DNA ligase |
| Storage | Handles and control hairpin −20 °C; STOP solution at room temperature |
| Buffers (not supplied) | Tethering, channels 1–2: 10 mM Tris-HCl pH 8.0, 50 mM NaCl, 0.1 mM EDTA (optional) Measurement, channel 3: 10 mM Tris-HCl pH 8.0, 50 mM NaCl, 10 mM MgCl₂ |
| Price | $350 |
1 C-Trap run = 20 µl of stopped ligation in 500 µl of tethering buffer (channel 2).