Home / Optical tweezers / 6.3 kb Handles for dsDNA
Two biotinylated handles. Ligate any dsDNA insert between them (Golden Gate compatible).
An optional Atto dye on Handle L or R (7 bp from the overhang) marks the tether direction.
Fluorophore: no dye · L – Atto647N · R – Atto565, all in stock
Other dye? Ask for your fluorophore of choice →
Per kit: 8 reactions (up to 40 C-Trap runs)
$350 no dye · $390 with dye
| Kit contents | Handle L and Handle R: 40 ng/µl, 20 µl each Control DNA 5.3 kb: 25 ng/µl, 8 µl STOP solution 5×: 350 µl |
|---|---|
| Length | 6,286 bp each |
| Structure | 2 handles, your dsDNA insert ligated between (no size limit) |
| Overhangs | 5′-phosphorylated 4 nt overhangs: GTTG (L) and TGGT (R). Your insert needs 5′-phosphorylated CAAC and ACCA overhangs |
| End labels | 3× biotin, outer end of each handle |
| Dye position | Handle L or R, 7 bp from the overhang |
| Fluorophores | In stock: no dye, L – Atto647N (Ex/Em 646/664 nm), R – Atto565 (Ex/Em 563/592 nm) On request: Atto488, Atto565 or Atto647N on either handle |
| Per kit | 8 ligation reactions, 5 C-Trap runs each (up to 40) |
| Workflow | 1 h ligation at room temperature, add STOP solution, tether directly: no purification |
| Supplied by the user | T4 DNA ligase |
| Storage | Handles and Control DNA −20 °C; STOP solution at room temperature |
| Tethering buffer (not supplied) | 10 mM Tris-HCl pH 8.0, 50 mM NaCl, 0.1 mM EDTA (optional), channels 1–2 |
| Price | $350 no dye · $390 with dye |
1 C-Trap run = 10 µl of stopped ligation in 500 µl of tethering buffer (channel 2).